Data/hora: 01/06/2024 07:46:12

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium	
Autores: 	Ben Khedher,Saoussen; Jaoua,Samir; Zouari,Nabil.	
Data: 	2013-09-01	
Ano: 	2013	
Palavras-chave: 	Sporeless Bacillus thuringiensis; Delta-endotoxins; Cheap medium; Optimisation; Response surface methodology.	
Resumo: 	In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L-1 starch, 30 g L-1 soya bean and 9g L-1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300043	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822013000300043	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.44 n.3 2013	
Fonte: 	info:eu-repo/semantics/openAccess